Gene description for PECAM1
Gene name platelet and endothelial cell adhesion molecule 1
Gene symbol PECAM1
Other names/aliases CD31
CD31/EndoCAM
GPIIA'
PECA1
PECAM-1
endoCAM
Species Homo sapiens
 Experiment description of studies that identified PECAM1 in Endothelial cells

1
Experiment ID223
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method FACS
Western blotting
PubMed ID 10393703   
OrganismHomo sapiens
Experiment descriptionIn vitro generation of endothelial microparticles and possible prothrombotic activity in patients with lupus anticoagulant
AuthorsCombes V, Simon AC, Grau GE, Arnoux D, Camoin L, Sabatier F, Mutin M, Sanmarco M, Sampol J, Dignat-George F
Journal name J Clin Invest
Publication year1999
SampleEndothelial cells
Sample nameHUVECS
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
Western blotting
EV-TRACK -

2
Experiment ID267
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method FACS
PubMed ID 12757771   
OrganismHomo sapiens
Experiment descriptionEndothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis
AuthorsJimenez JJ, Jy W, Mauro LM, Soderland C, Horstman LL, Ahn YS
Journal name Thromb Res
Publication year2003
SampleEndothelial cells
Sample nameBrain microvascular endothelial cells - ACBRI 376 - Control
TNF alpha
GFD
Isolation/purification methodsDifferential centrifugation
FACS
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK -

3
Experiment ID268
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method FACS
PubMed ID 12757771   
OrganismHomo sapiens
Experiment descriptionEndothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis
AuthorsJimenez JJ, Jy W, Mauro LM, Soderland C, Horstman LL, Ahn YS
Journal name Thromb Res
Publication year2003
SampleEndothelial cells
Sample nameGlomerular microvascular endothelial cells - ACBRI 128 - Control
TNF alpha
GFD
Isolation/purification methodsDifferential centrifugation
FACS
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK -

4
Experiment ID269
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method FACS
PubMed ID 12757771   
OrganismHomo sapiens
Experiment descriptionEndothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis
AuthorsJimenez JJ, Jy W, Mauro LM, Soderland C, Horstman LL, Ahn YS
Journal name Thromb Res
Publication year2003
SampleEndothelial cells
Sample nameCoronary artery endothelial cells - ACBRI 377 - Control
TNF alpha
GFD
Isolation/purification methodsDifferential centrifugation
FACS
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyFACS
EV-TRACK -

5
Experiment ID385
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18563738   
OrganismHomo sapiens
Experiment descriptionComparative proteomic analysis of PAI-1 and TNF-alpha-derived endothelial microparticles.
AuthorsPeterson DB, Sander T, Kaul S, Wakim BT, Halligan B, Twigger S, Pritchard KA Jr, Oldham KT, Ou JS.
Journal name Proteomics
Publication year2008
SampleEndothelial cells
Sample nameEndothelial cells (HUVEC)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

6
Experiment ID386
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18563738   
OrganismHomo sapiens
Experiment descriptionComparative proteomic analysis of PAI-1 and TNF-alpha-derived endothelial microparticles.
AuthorsPeterson DB, Sander T, Kaul S, Wakim BT, Halligan B, Twigger S, Pritchard KA Jr, Oldham KT, Ou JS.
Journal name Proteomics
Publication year2008
SampleEndothelial cells
Sample nameTreated by PAI-1-Endothelial cell (HUVEC)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

7
Experiment ID387
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 18563738   
OrganismHomo sapiens
Experiment descriptionComparative proteomic analysis of PAI-1 and TNF-alpha-derived endothelial microparticles.
AuthorsPeterson DB, Sander T, Kaul S, Wakim BT, Halligan B, Twigger S, Pritchard KA Jr, Oldham KT, Ou JS.
Journal name Proteomics
Publication year2008
SampleEndothelial cells
Sample nameTreated by TNF-alpha-Endothelial cells (HUVEC)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

8
Experiment ID393
Identified moleculeprotein
Extracellular vesicle typeMicroparticles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 19369228   
OrganismHomo sapiens
Experiment descriptionProteomic analysis reveals presence of platelet microparticles in endothelial progenitor cell cultures.
AuthorsProkopi M, Pula G, Mayr U, Devue C, Gallagher J, Xiao Q, Boulanger CM, Westwood N, Urbich C, Willeit J, Steiner M, Breuss J, Xu Q, Kiechl S, Mayr M.
Journal name Blood
Publication year2009
SampleEndothelial cells
Sample nameNormal-Endothelial progenitor cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK -

9
Experiment ID502
Identified moleculeprotein
Extracellular vesicle typeMembrane vesicles/Extracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 23436686   
OrganismHomo sapiens
Experiment descriptionA comprehensive characterization of membrane vesicles released by autophagic human endothelial cells.
AuthorsPallet N, Sirois I, Bell C, Hanafi LA, Hamelin K, Dieudé M, Rondeau C, Thibault P, Desjardins M, Hebert MJ.
Journal name Proteomics
Publication year2013
SampleEndothelial cells
Sample nameSerum-starved-Human umbilical vascular endothelial cells
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV130048: EV-METRIC:44%

10
Experiment ID551
Identified moleculeprotein
Extracellular vesicle typeExosomes/Extracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 24009886   
OrganismHomo sapiens
Experiment descriptionCellular stress conditions are reflected in the protein and RNA content of endothelial cell-derived exosomes.
Authorsde Jong OG, Verhaar MC, Chen Y, Vader P, Gremmels H, Posthuma G, Schiffelers RM, Gucek M, van Balkom BW.
Journal name J Extracell Vesicles
Publication year2012
SampleEndothelial cells
Sample nameStressed (hypoxia, TNF-alpha-induced activation, high glucose and mannose concentrations)-Endothelial cells (HMEC-1)
Isolation/purification methods-
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
EV-TRACK EV120004: EV-METRIC:67%

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